Synthesis and in vitro studies of PLGA-DTX nanoconjugate as potential drug delivery vehicle for oral cancer

Parul Gupta,1 Manjri Singh,2 Rajiv Kumar,3 Jodi Belz,3 Rishi Shanker,4 Premendra D Dwivedi,1 Srinivas Sridhar,3 Surinder P Singh2 1CSIR-Indian Institute of Toxicological Research, Lucknow, 2CSIR-National Physical Laboratory, New Delhi, India; 3Nanomedicine Science and Technology Center, Northeastern University, Boston, MA, USA; 4Institute of Life Sciences, Ahmedabad University, Ahmedabad, India Abstract: Advances in nanotechnology have led to the design of multifunctional nanoparticles capable of cellular imaging, targeted drug delivery, and diagnostics for early cancer detection. We synthesized poly(lactic-co-glycolic acid) nanoparticles encapsulating a model radiosensitizing drug docetaxel accomplishing localized in situ delivery of the sensitizer to the tumor site. The synthesized nanoparticles have been characterized for their physicochemical properties. The in vitro cytotoxicity of drug-loaded nanoparticles has been studied on human tongue carcinoma cell line SCC-9 (ATCC-CRL-1629). Keywords: docetaxel, PLGA nanoformulatio