Effect of Vitamin E and Selenium on Oxidative Stress and Tissue Damages Induced by Electromagnetic Fields in Immature Mice Ovarian

Objective: Electromagnetic fields (EMF) due to increased oxidative stress impact on body systems including female reproductive system and reduce fertility. The use of antioxidants decreases the amount of damage through the oxidative stress reduction. The present research studies the effect of vitamin E and selenium on oxidative stress factors level resulting from EMF in immature mice ovarian tissue. Material and Methods: In this study, 48 immature mice were randomly divided into 6 groups (n=8): (1) control, (2) EMF, (3) EMF with Vitamin E, (4) EMF with selenium, (5) EMF with vitamin E and selenium and (6) vitamin E and selenium. Finally, after 2 months the mice were killed by cervical dislocation and the ovaries were removed to evaluate tissue damages and oxidative stress factors including malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD) and total antioxidant (TAC). Results: In mice under EMF, MDA level increased and SOD, GPX and TAC decreased in ovarian tissue (P <0.05). Treatment of mice under EMF via vitamin E with selenium and by vitamin E alone significantly improved these parameters (P