Effects of low temperature on the transgenic rapeseed plants (Brassica napus L.) with transgene of trans-factor OsMyb4 from rice

The effects of cold stress on contents of proline, malondialdehyde (MDA), soluble sugars, enzyme activity of Superoxide Dismutase (SOD) and peroxidase investigated in transgenic rapeseed plants (Brassica napus L.) which contain gene OsMyb4 and non-transgenic plants. Under the cold stress sugar content in transgenic plants was significantly lower than in non-transformed, however the proline content was higher. MDA content was increased in non-transgenic plants and was decreased in the transgenic. That the change in the activity of the SOD enzyme in the both lines of the plants was similar, and peroxidase activity for 2 days under cold stress in non-transgenic plants increased, remaining constant at the transgenic, but in 5 days decreased in both lines of plants. After the return of plants to +24 °C, all parameters in all plant lines returned to normal. According to the results of experiments suggests that the kind of protein content for expressing transgenic plants OSMYB4 affects the other genes, low and high expression