This paper provides a brief discussion of the history of modern statistics, taking into account the contributions of Fisher (1945). Focuses on, discuss the emergence of ANOVA (analysis of variance - ANOVA) and multiple comparison method, and at the same time, talk about the verification of the assumptions of the mathematical models in a completely randomized design and in blocks. The verification of assumptions will be through commands that trigger additional package features free software "R version 3.2.3." data from one experiment were used "tomato seedling production (Solanum lycopersicum) on different substrates" for discussion. The same was conducted at the Federal Institute Goiano - Morrinhos Campus, and the design adopted was randomi...