Abstract Background Several fungi-specific primers target the 18S rRNA gene sequence, one of the prominent markers for fungal classification. The design of most primers goes back to the last decades. Since then, the number of sequences in public databases increased leading to the discovery of new fungal groups and changes in fungal taxonomy. However, no reevaluation of primers was carried out and relevant information on most primers is missing. With this study, we aimed to develop an 18S rRNA gene sequence primer toolkit allowing an easy selection of the best primer pair appropriate for different sequencing platforms, research aims (biodiversity assessment versus isolate classification) and target groups. Results We performed an intensive l...
The aim of this study was to assess potential candidate gene regions and corresponding universal pri...
The kingdom Fungi is estimated to include 1.5 million or more species, playing key roles as decompos...
The aim of this study was to assess potential candidate gene regions and corresponding universal pri...
BACKGROUND: Several fungi-specific primers target the 18S rRNA gene sequence, one of the prominent m...
List of the three newly designed primer pairs passing the evaluation criteria. Primer performance wa...
Primer pairs suitable for the amplification of specific fungal phyla/subphyla. Characteristics of th...
Performance of the most promising primer pairs empirically tested on 12 fungal strains. (XLSX 10 kb
List of the designed annealing blocking oligonucleotides for the eukaryotic groups Stramenopiles, Al...
<div><p>High-throughput sequencing of ribosomal RNA gene (rDNA) amplicons has opened up the door to ...
List of the 164 fungi-specific primers detected by a literature research. For each primer, performan...
Taxonomic composition of three environmental samples. Barchart indicates relative sequence abundance...
List of the seven most promising primer pairs for biodiversity assessments identified by in silico P...
Metabarcoding has become an important tool in the discovery of biodiversity, including fungi, which ...
Target-gene amplicon sequencing is the most exploited high-throughput sequencing application in micr...
The usage of molecular phylogenetic approaches is critical to advance the understanding of systemati...
The aim of this study was to assess potential candidate gene regions and corresponding universal pri...
The kingdom Fungi is estimated to include 1.5 million or more species, playing key roles as decompos...
The aim of this study was to assess potential candidate gene regions and corresponding universal pri...
BACKGROUND: Several fungi-specific primers target the 18S rRNA gene sequence, one of the prominent m...
List of the three newly designed primer pairs passing the evaluation criteria. Primer performance wa...
Primer pairs suitable for the amplification of specific fungal phyla/subphyla. Characteristics of th...
Performance of the most promising primer pairs empirically tested on 12 fungal strains. (XLSX 10 kb
List of the designed annealing blocking oligonucleotides for the eukaryotic groups Stramenopiles, Al...
<div><p>High-throughput sequencing of ribosomal RNA gene (rDNA) amplicons has opened up the door to ...
List of the 164 fungi-specific primers detected by a literature research. For each primer, performan...
Taxonomic composition of three environmental samples. Barchart indicates relative sequence abundance...
List of the seven most promising primer pairs for biodiversity assessments identified by in silico P...
Metabarcoding has become an important tool in the discovery of biodiversity, including fungi, which ...
Target-gene amplicon sequencing is the most exploited high-throughput sequencing application in micr...
The usage of molecular phylogenetic approaches is critical to advance the understanding of systemati...
The aim of this study was to assess potential candidate gene regions and corresponding universal pri...
The kingdom Fungi is estimated to include 1.5 million or more species, playing key roles as decompos...
The aim of this study was to assess potential candidate gene regions and corresponding universal pri...