Pathogens Identification and Evaluation of Nigella sativa’s (Kalonji) Antibacterial Activity

To examine the presence of different pathogens capable of causing diseases in the humans, bacteria were isolated from clinical samples. 40 different clinical samples were collected from which different pathogens were isolated and identified by using conventional methods. In this article we also checked the antibacterial activity of Nigella sativa. The Nigella sativa commonly known as kalonji are use in herbal medicine, throught the world for the prevention and treatment of many diseases. The kalonji oil has many properties like antibacterial, antiparasitic, antioxidant etc. kalonji oil was used to study the antibacterial activity against 5 clinical isolates of bacteria. Disc diffusion technique was applied on inoculated Muellar Hinton agar plates. Among Gram positive bacteria only Staphylococcus aureus was used, Among Gram negative bacteria Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus, and E.coli were used. The oil showe