Comparative evaluation of the effect of commercially available two different forms of denture cleansers on denture biofilm in diabetic and nondiabetic individuals: An in vivo study

Statement of Problem: Denture cleansers are known to decrease the microbial counts in denture wearers. In certain medical conditions such as in diabetes, achieving reduction in microbial count and maintaining proper denture hygiene is difficult. Denture cleansers are used as an aid in maintaining denture hygiene in such patients. A number of tablets, pastes, and powders are available for cleaning dentures. Although the comparison of chemical methods and brushing is relatively common, their difference in diabetic denture biofilm and nondiabetic denture biofilm has not been previously described. Purpose: The purpose of this study was to investigate the effect of commercially available two different forms of denture cleansers on the denture biofilm in diabetic and nondiabetic denture-wearing individuals. Methodology: Twenty diabetic denture and 20 nondiabetic denture wearers were randomly selected for the study. Denture biofilm was collected twice for each participant, before and after use of denture cleanser for 21-day period. For sample collection, complete dentures were rinsed with distilled water and brushed. Inoculation was done with sterile loop and cultured on blood agar and chrome agar. Blood agar plates were incubated at 370°C for 24 h and chrome agar at 290°C for 24 h. Colonies' characteristics of the Streptococcus species and Candida species were counted after the incubation period. Results: The results showed a statistically significant reduction in the colony-forming unit (CFU) counts after the usage of denture cleansers. Furthermore, the Candidal count difference obtained by the use of effervescent tablets was more than paste form. Conclusions: The present study concluded that microbial colonization count of diabetic and nondiabetic denture biofilm significantly reduced after 21 days of usage of denture cleansers, and the effervescent tablets were more effective against Candida than paste form