Molecular identification and investigations of contagious ecthyma (Orf virus) in small ruminants, North west Ethiopia

Abstract Background Orf virus, the prototype of parapoxvirus, is the main causative agent of contagious ecthyma. Little is known about the status of the disease in Ethiopia and this study was aimed at determining its status using PCR as a confirmatory tool. Methods a total of 400 randomly selected sheep and goat was screened for the identification of the virus using amplification of B2L gene and transfection of mammalian cells (VERO cells). Results Out of 400 animals screened for infection of the virus, 48 animals were found positive to PCR and revealed an overall incidence of 12%. Different epidemiological parameters were considered to look at the association with incidence of the disease and of which, only species of the animal(sheep), non-vaccinated and non-treated animals, nursing animals, poor body condition animals, extensively managed animals, animals having mouth lesion, and study areas having outbreak history showed higher prevalence. A univariate logistic regression analysis showed statistically significant difference in all variables (P < 0.05). Whereas, age and sex of animals showed no significant difference (P < 0.05). Conclusion The result of the present finding showed high incidence of Orf virus in the region as confirmed through PCR