Protection of mice against experimental infection wild Brucella abortus strain by vaccination via oral and intraperitoneal routes with Brucella abortus RB51

The study was designed to detect the effect of an oral and intraperitoneal (I/P) immunization of mice with B. Abortus RB51 to protect mice against a wild strain of B. abortus I/P challenge infection. Three groups of mice were used in this study. The first group (1st G) immunized with 108 *2 CFU of B. Abortus RB51 intraperitoneally (I/P). The second group (2nd G) immunized orally with 108 *2 CFU dose (Ten minutes prior immunization, all mice were drenched with 0.2 ml of 10% sodium bicarbonate to neutralize gastric acidity). Whereas, the third group (3rdgroup) inoculated with 0.2 ml phosphate buffer saline (PBS) and acted as the control group. The results indicated that there was a significant difference (P