Effects of Carob (Ceratonia siliqua) on Sperm Quality, Testicular Structure, Testosterone Level and Oxidative Stress in Busulfan-Induced Infertile Mice

Background: Ceratonia silique has antioxidant activities that may inactivate toxic factors and influence sperm quality. To the best of the authors’ knowledge, there is no available data on the effects of carob on male fertility. Hence, the purpose of this study was to investigate the effects of carob on sperm quality, testicular structure, and level of testosterone hormone in busulfan-induced infertile mice. Methods: Sixty-four adult male mice were randomly divided into 8 groups (control, sham, busulfan and carobs 1 to 5). The busulfan group was injected a single dose of 10 mg/kg busulfan intraperitoneally. Carobs 1 to 5 groups received intraperitoneal doses of 800, 400, 200, 100 and 50 mg/kg of carob extract plus a single dose of 10 mg/kg busulfan for 35 days. The sperm analysis, morphometric study, testosterone levels and oxidative stress determination were done on the 35th day of the experiment. Results: The lowest percentage of sperm parameters was related to the busulfan group and the highest was related to the carobs 1 and 2 groups. The seminal vesicles index of the carob 1 group showed a significant increase as compared to the busulfan group (p < 0.001). A significant increase was observed in the mean value of germinal epithelium thickness, as well as thiol and catalase levels in carobs 1 and 2 groups as compared to the busulfan group (p < 0.001). There was a significant increase in the mean level of testosterone in the carob groups as compared to the busulfan group (p < 0.001). Also, there was a significant decrease in the mean value of malondialdehyde level in the carobs 1 and 2 groups p < 0.001) and a significant increase in the mean value of superoxide dismutase enzyme in the carob groups as compared to the busulfan group (p < 0.001). Conclusion: Administration of 800 mg/kg of carob extract for 35 days improved sperm quality, biochemical parameters, thickness of germinal epithelium and testosterone levels in infertile mice induced by busulfan