Expression of RecF and GyrB Genes in a Low Ciprofloxacin-resistant ‎Escherichia coli Mutant

Introduction: Replication and recombination are two fundamental cellular processes. GyrB and RecF are two proteins that are involved in these processes, respectively. GyrB is a subunit of DNA gyrase, the enzyme maintains negative supercoil in genomic DNA. RecF helps to load RecA in a single-strand gap, which is then repaired by RecA. Genes encoding these proteins are located in the same operon. Besides the general promoter, which is used in logarithmic phase, RecF has its own promoter, which is activated in stationary phase. The aims of this study were to investigate the expression of these genes and to estimate the pattern of RecF expression in the presence of ciprofloxacin. Materials and Methods: RNAs of the cells were first extracted in logarithmic phase and after cDNA synthesis, the relative expression of these genes was determined in the E. coli mutant with low resistance to ciprofloxacin by Real-Time PCR. Results: Results showed that both genes are overexpressed similarly in the mutant cell treated with a low amount of ciprofloxacin in the exponential phase of growth. Conclusion: The present work revealed that the variety and the number of the Cyanobacteria in this desert area are dependent on different parameters such as weather, humidity, source of nutrients, the amount of light absorbed by the surface and the temperature. The results of molecular identification validated the results of morphological tests