Effect of aqueous green tea extract on male Wistar rats reproductive hormones level

The current study was carried to investigate the effect of green tea extract in improvement of male rats reproductive efficiency after exposure to oxidative stress by streptozotocin.40 male rats at 60 days old with 135±11gm in weight were randomly divided into four equal groups, the first drenched with distilled water for 60 days as control group(C). the second (T1)was given aqueous extract of green tea (100mg/kg/BW) for 60 days, while the third group (T2 injected (i.p) single dose of streptozotocin (60mg/kg/BW) for induction of oxidative stress, the fourth group injected with single dose of streptozotocin (60mg/kg/BW) and after 30 days drenched with green tea extract(100mg/kg/BW) for 30 days.at day60 of the experiment all animals were sacrificed, blood samples were collected from the ventral vein and serum samples were isolated for measurement of male reproductive hormones (LH, FSH, and Testosterone) by ELISA test. the testes samples were taken for histological study and dimensions of seminiferous tubules, samples of epididymis for the study of seminiferous tubules dimensions. The results of the study were revealed significant increase (P≥0.05)in testosterone and FSH in (T1) group compared with other groups while there are non-significant changes in LH concentration compared with other group