In ligand-protein binding experiments the major challenge is to separate bound from free ligand. Equilibrium and gel filtration separation techniques are often hampered by competition for the ligand and non-specific binding. Biophysical assays have attempted to circumvent this problem using titration calorimetry and spectroscopic methods. However, insoluble ligands require solvents that can overwhelm the discernible enthalpic changes of the protein and ligands. Spectroscopic methods are effective but may suffer from insensitivity (NMR) or the need for a lipid analog e.g., fluorescence and electron paramagnetic resonance. Our purpose is to compare the standard fluorescence assay to a technique we call high speed centrifugal precipitation. Hi...
Drug development is impeded by the need to design for each drug target a test that detects the bindi...
Kinetics of binding of dyes at different sites of human serum albumin (HSA) has been studied by sing...
Steady state and time resolved fluorometric and circular dichroism (CD) techniques have been exploit...
Rapid identification of small molecules that interact with protein targets using a generic screening...
Determination of binding affinity and thermodynamic parameters of interactions between two molecules...
<div><p>Sedimentation velocity analytical ultracentrifugation (SV) is a powerful first-principle tec...
Drug development is impeded by the need to design for each drug target a test that detects the bindi...
This unit provides guidelines on how to use steady-state fluorescence spectroscopy for the quantific...
<p>Currently there is a dearth of analytical techniques for studying protein-ligand interactions on ...
The binding of a drug or other ligand to plasma proteins can effect their absorption, metabolism and...
The biopharmaceutical industry is constantly developing biological drugs, resulting in increased lev...
AbstractChemical denaturant titrations can be used to accurately determine protein stability. Howeve...
In this work we return to the problem of the determination of ligand–receptor binding stoichiometry ...
Information about the stability of proteins is paramount to determine their optimal storage or react...
Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter droplet...
Drug development is impeded by the need to design for each drug target a test that detects the bindi...
Kinetics of binding of dyes at different sites of human serum albumin (HSA) has been studied by sing...
Steady state and time resolved fluorometric and circular dichroism (CD) techniques have been exploit...
Rapid identification of small molecules that interact with protein targets using a generic screening...
Determination of binding affinity and thermodynamic parameters of interactions between two molecules...
<div><p>Sedimentation velocity analytical ultracentrifugation (SV) is a powerful first-principle tec...
Drug development is impeded by the need to design for each drug target a test that detects the bindi...
This unit provides guidelines on how to use steady-state fluorescence spectroscopy for the quantific...
<p>Currently there is a dearth of analytical techniques for studying protein-ligand interactions on ...
The binding of a drug or other ligand to plasma proteins can effect their absorption, metabolism and...
The biopharmaceutical industry is constantly developing biological drugs, resulting in increased lev...
AbstractChemical denaturant titrations can be used to accurately determine protein stability. Howeve...
In this work we return to the problem of the determination of ligand–receptor binding stoichiometry ...
Information about the stability of proteins is paramount to determine their optimal storage or react...
Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter droplet...
Drug development is impeded by the need to design for each drug target a test that detects the bindi...
Kinetics of binding of dyes at different sites of human serum albumin (HSA) has been studied by sing...
Steady state and time resolved fluorometric and circular dichroism (CD) techniques have been exploit...