Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
- Katerina Vlahos
- Koula Sourris
- Robyn Mayberry
- Penelope McDonald
- Freya F. Bruveris
- Jacqueline V. Schiesser
- Kiymet Bozaoglu
- Paul J. Lockhart
- Edouard G. Stanley
- Andrew G. Elefanty
Publication date
January 2019
DOI Publisher
Elsevier BV Journal
Stem Cell ResearchAbstract
Abstract: We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms
Add to ORKG