Transport of Gold Nanoparticles by Vascular Endothelium from Different Human Tissues.
- Radka Gromnicova
- Mehmet Kaya
- Ignacio A Romero
- Phil Williams
- Simon Satchell
- Basil Sharrack
- David Male
DOIAbstract
The selective entry of nanoparticles into target tissues is the key factor which determines their tissue distribution. Entry is primarily controlled by microvascular endothelial cells, which have tissue-specific properties. This study investigated the cellular properties involved in selective transport of gold nanoparticles (<5 nm) coated with PEG-amine/galactose in two different human vascular endothelia. Kidney endothelium (ciGENC) showed higher uptake of these nanoparticles than brain endothelium (hCMEC/D3), reflecting their biodistribution in vivo. Nanoparticle uptake and subcellular localisation was quantified by transmission electron microscopy. The rate of internalisation was approximately 4x higher in kidney endothelium than brain e...
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