Identification of chemical compounds in hexane extractives of black locust heartwood by GC/MS method

Black locust wood is durable against microorganisms. The species is planted as a nitrogen fixing specie in many arid and semiarid areas of Iran. Extraction procedures included soaking wood samples in hexane solvent for 15 days were applied. Average percentage of black locust heartwood extractives was determined as 8.4 percent. Identification of chemical compounds in the extractives was performed on a GC/MS device. Generally, 25 compounds were identified with different percentage in heartwood. The most abundant compound within the hexane heartwood extract of black locust samples was hexadecanoic acid as a trimethylsilyl ester, amounting to 13.39% saturated fatty acids group. The most important identified unsaturated fatty acids in the heartwood of the species 9, 12-octadecadienoic acid (Z, Z) - (10.10%). Tetradecane (6.88%) and hexadecane (6.15%) were the most important identified alkenes in the heartwood of the species. Also squalene as pure and mineral hydrocarbon oil was identified amounting to 1.17%. Squalene Vaccine manufacturers has proved that the squalene is effective against virus diseases of animal