Evaluation of Caffeic Acid Phenethyl Ester Administration in Chronically Stressed Rats with Experimental Periodontitis.

Objectives: The aim of the present study was to investigate the therapeutic effects of systemic caffeic acid phenethyl ester treatment on oxidative stress and alveolar bone destruction in lipopolysaccharide (LPS)-induced periodontitis in chronically stressed rats.Materials and Methods: Fourty male Sprague Dawley rats were divided into four groups: 1) control group, 2) experimental periodontitis (EP), 3) EP and chronic stress (CS) group (EP-CS), and 4) EP-CS treated with CAPE (EP-CS-CAPE). To induce periodontitis, LPS was administered into the buccal gingiva of the test groups, and pure saline was administered for the control group. Two test groups were exposed to restraint stress and one group of these groups was treated with only a single dose of CAPE (10 mmol/kg). Likewise, saline was administered in the control, EP, and EP-CS groups. After 14 days, serum samples were collected from the heart, and all rats were sacrificed for analyses. Oxidative stress and interleukin (IL)-1β were investigated. The receptor activator of the nuclear factor kappa B ligand (RANKL) and alveolar bone loss were determined by immunohistochemical analysisResults: The oxidative stress, alveolar bone loss, IL-1β and RANKL levels were found significantly higher in the EP-CS group compared with control and EP groups (p<0.05). However, the administration of CAPE significantly reduced oxidative stress and IL-1β in the EP-CS-CAPE group compared with the EP-CS group (p<0.05). Also, CAPE treatment significantly reduced RANKL and alveolar bone loss in the EP-CS-CAPE group compared with the EP-CS group (p<0.05)Conclusions: The present results indicated that CAPE may inhibit alveolar bone loss by modulating the immune response and inflammatory process