Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs)

Summary: Background: Stem cell-derived by conditioned medium has potential as regenerative agent of skin cells because it contains many growth factors, cytokines and other regenerative biomolecules. Conditioned medium (CM) from human adipose tissue-derived MSCs (CM-hATMSCs) also has been known enriched growth factors that play an important role in epithelial wound repair, reduce wrinkles, and enhance wound healing. Objective: This study was performed to evaluate growth factors in CM such as Transforming Growth Factor (TGF)-β1, TGF-β2, Vascular Endothelial Growth Factor (VEGF)-2, Vascular Cell Adhesion Molecule (VCAM) 1, Epidermal Growth Factor (EGF), Fibroblast Growth Factor (FGF) in CM-hATMSCs treated with Fresh Frozen Plasma (FFP) and non-FFP at various passages. Methods: This study used ELISA method to measure the growth factors in CM-hATMSCs. Results: FGF was the highest modulator among FFP and non-FFP-treated cells at many passages compared to the others growth factor. Both FFP and non-FFP-treated cells showed significant difference (P < 0.05) in some growth factors. However, there was no significant differences in TGF-β1 at passage 3,7,11 and 15 in FFP-treated cells and non-FFP-treated cells. Conclusion: In summary, the highest concentration level found in CM-hATMSCs is FGF both in FFP and non-FFP-treated cells. FGF as growth factor composition in CM-hATMSCs has potential as wound healing and regeneration of cell and it can be used in anti-aging products. Keywords: Conditioned medium, Human adipose tissue derived-mesenchymal stem cells, Growth factor, Fresh frozen plasma, Wound healin