Influence of ethanolic extract of strawberry leaves for abrogating bromate hazards in male rats

Abstract Background Potassium bromate (KBrO3) has been used widely for water disinfection, hair coloring, oxidizing agent in food, food additive in bread making process as maturing agent, and as dough conditioner for flour. Purpose This study was carried out to investigate the effects of two doses (150 and 300 mg/kg) of ethanolic extract of strawberry leaves on antioxidant capacity of liver, kidney and testis, thyroid hormones, and kidney function in rats treated with potassium bromate as toxic and free radical producer compound. Study design Forty-eight male rats were divided: group 1 served as control, group 2 treated with oral dose of strawberry leaves extract (150 mg/kg), group 3 treated with oral dose of strawberry leaves extract (300 mg/kg), group 4 treated with oral dose of potassium bromate (20 mg/kg), group 5 treated with strawberry leaves extract (150 mg/kg) and potassium bromate (20 mg/kg), and group 6 treated with strawberry leaves extract (300 mg/kg) and potassium bromate (20 mg/kg) for 4 weeks. Methods Determination of thiobarbituric acid reactive substance (TBARS), glutathione (GSH), and superoxide dismutase (SOD) in liver, kidney, and testis tissues. Measurements of serum total protein, albumin, urea, creatinine, free triiodothyronine (FT3), free thyroxine (FT4), and insulin. Results Administration of potassium bromate (KBrO3) induced significant increase in TBARS; decrease in GSH and SOD in liver, kidney, and testis tissues; non-significant decrease in total protein and albumin; significant increase in serum urea, creatinine, and FT3; and significant decrease in FT4 and insulin. Administration of strawberry leaves extract showed significant improvement in some studied parameters. Conclusion In conclusion, the protective effect of strawberry leaves extract may be attributed to its antioxidant property