Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli

Catalina López-Saucedo
Jorge F. Cerna
Nicolas Villegas-Sepulveda
Rocío Thompson
F. Raul Velazquez
Javier Torres
Phillip I. Tarr
Teresa Estrada-García

Open link

Publication date

January 2003

DOI

10.3201/eid0901.010507

Publisher

Centers for Disease Control and Prevention (CDC)

ISSN

1080-6040

Journal

Emerging Infectious Diseases

Citation count (estimate)

127

Abstract

We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga-toxin–producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances