Delivery of osteogenesis-promoting microRNAs (miRNAs) is a promising approach to enhance bone regeneration. In this study, we generated nanocomplexes comprising the novel cell-penetrating peptide R9-LK15 and miR-29b and investigated their effects on osteogenic differentiation of bone mesenchymal stem cells (BMSCs). R9-LK15/miR-29b nanocomplexes were prepared and characterized. The transfection efficiency, cell viability, and osteogenic differentiation were investigated. The results showed that R9-LK15 maintained the stability of miR-29b in serum for up to 24 h. Moreover, R9-LK15 efficiently delivered miR-29b into BMSCs; the transfection efficiency was ~10-fold higher than that achieved using Lipofectamine 2000. The Cell Counting Kit-8 assay...
Osteoclasts are large multinucleated bone cells of the myeloid lineage. Osteoclasts secrete protease...
Cell-based tissue engineering strategies have shown tremendous promise for the repair of bone mass d...
According to recent discoveries from various metabolic disease studies, gene therapy is a fantastic ...
Abstract Background The restoration of the functional ability of mesenchymal stem cells (MSCs) using...
Patients diagnosed with osteosarcoma undergo extensive surgical intervention and chemotherapy result...
PURPOSE: Peptide-15 (P-15) is an analog of the cell-binding domain of collagen. P-15 has been shown ...
PURPOSE: Peptide-15 (P-15) is an analog of the cell-binding domain of collagen. P-15 has been shown ...
Mingxiang Cai,1,* Li Yang,2,* Shufan Zhang,1 Jiafan Liu,2 Yao Sun,1 Xiaogang Wang2 1Engineering Rese...
Dysregulated microRNAs in osteoclasts could cause many skeletal diseases. The therapeutic manipulati...
<div><p>Mesenchymal stem cells are multipotent adult stem cells capable of generating bone, cartilag...
Very large bone defects significantly diminish the vascular, blood, and nutrient supply to the injur...
Osteoporosis (OP) affects millions worldwide but currently cannot be cured. Suppressing the level of...
Although bone has an intrinsic capacity for self repair, the healing of large bone defects that typi...
Antiosteoporosis gene-based drug development strategies are presently focused on targeting osteoblas...
The molecular mechanisms how bone marrow-derived mesenchymal stem cells (BMSCs) differentiate into o...
Osteoclasts are large multinucleated bone cells of the myeloid lineage. Osteoclasts secrete protease...
Cell-based tissue engineering strategies have shown tremendous promise for the repair of bone mass d...
According to recent discoveries from various metabolic disease studies, gene therapy is a fantastic ...
Abstract Background The restoration of the functional ability of mesenchymal stem cells (MSCs) using...
Patients diagnosed with osteosarcoma undergo extensive surgical intervention and chemotherapy result...
PURPOSE: Peptide-15 (P-15) is an analog of the cell-binding domain of collagen. P-15 has been shown ...
PURPOSE: Peptide-15 (P-15) is an analog of the cell-binding domain of collagen. P-15 has been shown ...
Mingxiang Cai,1,* Li Yang,2,* Shufan Zhang,1 Jiafan Liu,2 Yao Sun,1 Xiaogang Wang2 1Engineering Rese...
Dysregulated microRNAs in osteoclasts could cause many skeletal diseases. The therapeutic manipulati...
<div><p>Mesenchymal stem cells are multipotent adult stem cells capable of generating bone, cartilag...
Very large bone defects significantly diminish the vascular, blood, and nutrient supply to the injur...
Osteoporosis (OP) affects millions worldwide but currently cannot be cured. Suppressing the level of...
Although bone has an intrinsic capacity for self repair, the healing of large bone defects that typi...
Antiosteoporosis gene-based drug development strategies are presently focused on targeting osteoblas...
The molecular mechanisms how bone marrow-derived mesenchymal stem cells (BMSCs) differentiate into o...
Osteoclasts are large multinucleated bone cells of the myeloid lineage. Osteoclasts secrete protease...
Cell-based tissue engineering strategies have shown tremendous promise for the repair of bone mass d...
According to recent discoveries from various metabolic disease studies, gene therapy is a fantastic ...