Survey of heavy metals (Pb & Hg) in cultured shrimp (Penaeus indicus) in Iran

The aim of this study was recognition of pollution, which is polluted by heavy metals (Lead and mercury). This study was carried out with cooperation of France Food Agency and AFSSA according to the latest international and Europe Union standards, for two years during 2000-2001. The sampling stages were done according to the European Union enclosed No. 4 in guide direction 96/23 by method to once time sampling, which was collected per 100 tons shrimp production. So, 42 sample in a year and were connected 84 samples totally during two years that the sample provided for each one was 500g. All samples were frozen and then were sent to France and AFSSA for analyzing. To study of heavy metals mercury and lead, in samples methods of chromatography with high efficiency HPLC and LC-MS and GC-MASS have been used. Quantitative determination rate in Pb was 20 and for Hg was 10µg /kg. The rest amount of this metals which cause we start our effort for controlling Pb and Mg pollution was found 500µg/kg. In this study, the quantitative determination rate of all samples as measured less than 500µg