LncRNA MIR100HG promotes cancer cell proliferation, migration and invasion in laryngeal squamous cell carcinoma through the downregulation of miR-204-5p

Yongyang Huang, Chao Zhang, Yanhui ZhouDepartment of ENT, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471000, People’s Republic of ChinaPurpose: LncRNA MIR100HG promotes several types of malignancies, while its involvement in other human diseases is unknown.Patients and methods: Our study included 70 patients with LSCC who were diagnosed and treated in the First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology from January 2016 to July 2018. qRT-PCR, cell transfection, in vitro cell proliferation assay, cell migration and invasion assay were applied for the research.Results: In the present study we found that MIR100HG was upregulated, while miR-204-5p was downregulated in tumor tissues than in adjacent healthy tissues of laryngeal squamous cell carcinoma (LSCC) patients. Expression of MIR100HG was significantly affected by AJCC stage. A significant and inverse correlation between MIR100HG and miR-204-5p was found in tumor tissues but not in adjacent healthy tissues of LSCC patients. Overexpression of MIR100HG resulted in the downregulation of miR-204-5p in LSCC cells, while miR-204-5p overexpression failed to significantly affect MIR100HG expression. Overexpression of MIR100HG led to promoted, while miR-204-5p, overexpression led to inhibited proliferation, migration and invasion of LSCC cells. In addition, miR-204-5p overexpression attenuated the enhancing effects of MIR100HG overexpression on cancer cell proliferation, migration and invasion.Conclusion: Therefore, lncRNA MIR100HG promoted cancer cell proliferation, migration and invasion in LSCC possibly through the downregulation of miR-204-5p.Keywords: laryngeal squamous cell carcinoma, lncRNA MIR100HG, miR-204-5