Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonuclease restriction for simultaneous detection of nucleic acids and elimination of carryover contamination. Reaction mixtures were detected in a nanoparticle-based lateral flow biosensor (LFB). The assay exhibited attractive traits in that it did not require the use of labeled primers or labeled probes, and thus, the technique could prevent undesired results arising from unwanted hybridization between labeled primers or between a probe and labeled primer. Isothermal amplification and endonuclease restriction digestion were conducted in a single pot, and the use of a closed-tube amplification removed false-positive results due to contaminants. To va...
Rapid, efficient and accurate nucleic acid molecule detection is important in the screening of disea...
Staphylococcus aureus (S. aureus), including methicillin-resistant S. aureus (MRSA), is one of the m...
Staphylococcus aureus (S. aureus) contamination in food safety has become a worldwide health problem...
Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonucleas...
Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonucleas...
Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonucleas...
Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonucleas...
In this study, a whole-course nucleic-acid-constructed biosensor that combines the all-in-one concep...
Nucleic acid lateral flow assays (NALFA) are often performed with gold nanoparticles. These are typi...
Nucleic acid detection is of central importance for the diagnosis and treatment of genetic diseases,...
Real time PCR is the mainstay of current nucleic acid assays, underpinning applications in forensic ...
2021 Summer.Includes bibliographical references.Rapid, accurate, reliable nucleic acid testing (NAT)...
The specific and multiplexed detection of DNA underpins many analytical methods, including the detec...
Amplification reactions coupled with electrophoresis or real-time fluorescence system have been succ...
Yi Wang,1 Hui Li,1,2 Yan Wang,1 Hua Li,1 Lijuan Luo,1 Jianguo Xu,1 Changyun Ye1 1State Key Laborato...
Rapid, efficient and accurate nucleic acid molecule detection is important in the screening of disea...
Staphylococcus aureus (S. aureus), including methicillin-resistant S. aureus (MRSA), is one of the m...
Staphylococcus aureus (S. aureus) contamination in food safety has become a worldwide health problem...
Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonucleas...
Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonucleas...
Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonucleas...
Here, we reported on a label-free cross-priming amplification (CPA) scheme that utilized endonucleas...
In this study, a whole-course nucleic-acid-constructed biosensor that combines the all-in-one concep...
Nucleic acid lateral flow assays (NALFA) are often performed with gold nanoparticles. These are typi...
Nucleic acid detection is of central importance for the diagnosis and treatment of genetic diseases,...
Real time PCR is the mainstay of current nucleic acid assays, underpinning applications in forensic ...
2021 Summer.Includes bibliographical references.Rapid, accurate, reliable nucleic acid testing (NAT)...
The specific and multiplexed detection of DNA underpins many analytical methods, including the detec...
Amplification reactions coupled with electrophoresis or real-time fluorescence system have been succ...
Yi Wang,1 Hui Li,1,2 Yan Wang,1 Hua Li,1 Lijuan Luo,1 Jianguo Xu,1 Changyun Ye1 1State Key Laborato...
Rapid, efficient and accurate nucleic acid molecule detection is important in the screening of disea...
Staphylococcus aureus (S. aureus), including methicillin-resistant S. aureus (MRSA), is one of the m...
Staphylococcus aureus (S. aureus) contamination in food safety has become a worldwide health problem...