Correlation of peritumoral edema and microvessel density with tissue expression of VEGF, semaphorins 3A and 3C in patients with meningioma

Background: Several angiogenic factors correlate with angiogenesis in meningioma while their exact role is yet to be identified. Semaphorins are described with a variety of physiological functions including angiogenesis and migration of neural crest cells. Objective. We aimed to determine the correlation of semaphorin 3A, 3C (Sema 3A and 3C) and vascular endothelial growth factor (VEGF) expression with microvessel density (MVD) and peritumoral brain edema (PTBE) in meningioma. Methods. In this study 21 patients with grade I meningioma were included. PTBE was measured on axial and coronal brain MRI. Tissue expression of semaphorin 3A, 3C and VEGF were determined using quantitative real-time PCR (qRT-PCR). Result. We found that tumor edema index was negatively associated with tissue expression of semaphorin 3C (r= –0.437, p=0.048). Sema 3A and VEGF did not show statistically significant correlation with tumor characteristics studied (p> 0.05). We also found that the mean vascular density of the menigiomas was positively associated with intra operative blood loss (r=0.503, p=0.010). Conclusion: Our data indicates an inverse correlation of Sema3C and peritumoral edema, while no correlations could be shown for Sema 3A and VEGF. Thus Sema3C may be identified as an appropriate inhibitor of pathological angiogenesis in human meningioma. However, confirmation of this finding in a larger dataset is warranted