Histopathology of mortality in cultured Rainbow trout fry in some coldwater hatcheries and fish farms of Iran

Causes of the mortality of cultured rainbow trout fry in coldwater hatcheries and fish farms in Iran were investigated. Some 104 tissue specimens of liver, kidney, spleen, pancreas, intestine, and gill from 59 diseased fries from Mazandaran, Fars, Markazy and Kohgiloyely Boyerahmad provinces were collected for histopathological studies. Tissues were immediately fixed in 10% buffered formalin for at least 24 hours. The fixed tissues were processed in an automatic tissue processor using standard procedure. After processing, tissues were embedded into wax and 5 micron sections were prepared using a rotary microtome. The sections were stained using II & E staining method, and examined under compound microscope. The affected fries were dark in color, and showed exophthalmia, ascitis, erratic swimming, faecal casts in the anal area and lethargy. They also gathered near the outlet of the ponds. Microscopic examination of the tissues revealed histopathologic changes including congestion, infla....