Optimization of tetrazolium tests to assess the quality of Platymiscium floribundum , Lonchocarpus muehlbergianus and Acacia polyphylla DC. Seeds

Abstract: The tetrazolium (TZ) test is an alternative to conventional germination tests, which usually require longer periods for seed quality evaluation. However, it is necessary to develop specific methodologies for each species, determining the concentration and time of exposure to salt. Thus, the objectives of this study were (i) to optimize the application of tetrazolium in forest seeds of Platymiscium floribundum Vog., Lonchocarpus muehlbergianus Hassl. and Acacia polyphylla DC., with the proposal of an evaluation protocol for tetrazolium test in order to reduce the subjectivity of interpretation and (ii) to adapt standardized methodologies to evaluate the seed quality of forest species using TZ. To perform the tests, the seeds were submitted to immersion times and concentrations (0.1, 0.5 and 1.0%) in solution of 2,3,5-triphenyl tetrazolium chloride. The ideal concentrations and immersion time for each species were: P. floribundum - 0.5 and 1.0% for 60 minutes; L. muehlbergianus - 0,1% for 6 hours and A. polyphylla - 0,5% for 4 hours. Each seed was evaluated by color, size and localization of colored spots in three zones of low, medium and significant impact on germinability and after classified in three vigor classes. The protocol and the proposed methodology presented high efficiency according to the established parameters