Magnetic separation and high reusability of chloroperoxidase entrapped in multi polysaccharide micro-supports

Trabajo presentado a la IV Reunión de Jóvenes Investigadores en Coloides e Interfases, celebrada en Córdoba del 7 al 9 de febrero de 2018.Enzyme immobilization on magnetic supports represents a great advantage for the industrial application of enzymatic catalysis since it allows an easy recovery of the catalyst, avoiding any contamination of the product by residual enzyme. Iron oxide nanoparticles are very useful for this purpose. They are frequently coated with polymers to diminish the interaction between the magnetic cores themselves, improving colloidal stability of the support, and preventing any interaction with the environment that would affect both support properties and enzyme stability. In this work different magnetic micro-supports, based on polydopammine-coated iron oxide nanoparticles with a multi polysaccharidic shell, have been developed. These supports have been used to immobilize chloroperoxidase, a very interesting enzyme, able to catalyze many reactions of large-scale interest, but whose application is limited by its sensitivity to reaction conditions. The multi polysaccharidic shells of the supports were obtained through a combination of chitosan and alginate, that were chosen for their pH-dependent properties and for their already reported stabilizing effect on this enzyme, especially in the case of chitosan. An in-depth analysis of physicochemical properties of all the developed magnetic supports, especially in terms of their stability under reaction conditions, allowed to find the one that showed the best reusability of the catalyst.Peer Reviewe