Imaging cytosolic calcium in neurons is emerging as a new tool in neurological disease diagnosis, drug screening, and toxicity testing. Ca2+ oscillation signatures show a significant variation depending on GPCR targeting agonists. Quantification of Ca2+ spike trains in ligand induced Ca2+ oscillations remains challenging due to their inherent heterogeneity in primary culture. Moreover, there is no framework available for identification of optimal number of clusters and distance metric to cluster Ca2+ spike trains. Using quantitative confocal imaging and clustering analysis, we show the characterization of Ca2+ spiking in GPCR targeting drug-treated primary culture of hippocampal neurons. A systematic framework for selection of the clusterin...
Calcium spiking can be used for drug screening studies in pharmaceutical industries. However, perfor...
Neuronal synchronization contributes to various cognitive functions and disruption in synchronicity ...
Genetically-encoded calcium indicators (GECIs) hold the promise of monitoring [Ca(2+)] in selected p...
Imaging cytosolic calcium in neurons is emerging as a new tool in neurological disease diagnosis, dr...
Imaging cytosolic calcium in neurons is emerging as a new tool in neurological disease diagnosis, dr...
Measurement of cytosolic calcium activity in single neuron and quantification of response to drug tr...
Glutamate is the primary fast excitatory neurotransmitter which is responsible for neuronal communic...
<p><b>a</b>, Confocal imaging of synchronously spiking glia-derived neurons (re-computing of earlier...
Current methods of drug screening and disease diagnosis are mainly based on biomarker information ob...
Advances in microscopy enable monitoring a broad spectrum of heterogeneity in calcium (Ca2+)-spike t...
Intracellular calcium spiking generates versatile signals that control various key neuronal function...
G protein-coupled receptors (GPCRs) are targets for designing a large fraction of the drugs in the p...
One of the major challenges in analyzing large scale intracellular calcium spiking data obtained thr...
Genetically-encoded calcium indicators (GECIs) hold the promise of monitoring [Ca(2+)] in selected p...
Calcium (Ca 2+ ) spiking in neurons generates versatile intracellular signals that control key funct...
Calcium spiking can be used for drug screening studies in pharmaceutical industries. However, perfor...
Neuronal synchronization contributes to various cognitive functions and disruption in synchronicity ...
Genetically-encoded calcium indicators (GECIs) hold the promise of monitoring [Ca(2+)] in selected p...
Imaging cytosolic calcium in neurons is emerging as a new tool in neurological disease diagnosis, dr...
Imaging cytosolic calcium in neurons is emerging as a new tool in neurological disease diagnosis, dr...
Measurement of cytosolic calcium activity in single neuron and quantification of response to drug tr...
Glutamate is the primary fast excitatory neurotransmitter which is responsible for neuronal communic...
<p><b>a</b>, Confocal imaging of synchronously spiking glia-derived neurons (re-computing of earlier...
Current methods of drug screening and disease diagnosis are mainly based on biomarker information ob...
Advances in microscopy enable monitoring a broad spectrum of heterogeneity in calcium (Ca2+)-spike t...
Intracellular calcium spiking generates versatile signals that control various key neuronal function...
G protein-coupled receptors (GPCRs) are targets for designing a large fraction of the drugs in the p...
One of the major challenges in analyzing large scale intracellular calcium spiking data obtained thr...
Genetically-encoded calcium indicators (GECIs) hold the promise of monitoring [Ca(2+)] in selected p...
Calcium (Ca 2+ ) spiking in neurons generates versatile intracellular signals that control key funct...
Calcium spiking can be used for drug screening studies in pharmaceutical industries. However, perfor...
Neuronal synchronization contributes to various cognitive functions and disruption in synchronicity ...
Genetically-encoded calcium indicators (GECIs) hold the promise of monitoring [Ca(2+)] in selected p...