Absence of glycogen cell precursors in <i>Ascl2</i> mutant placentae.

(A) Histological sections of wild-type and rescued Ascl2KO/Del7AI placentae at E16.5 were stained with heamatoxylin and eosin (H&E, top) or PAS with heamatoxylin nuclear counter-stain (bottom). SpT, spongiotrophoblast cells; lab, labyrinthine layer; GlyT, glycogen trophoblast cells; P-TGC, parietal trophoblast giant cells. Scale bar: 0.5 mm. (B) Immunofluorescence staining with PCDH12 polyclonal antibodies (red) and phalloidin (green) on cryosections of E8.5 wild-type and Ascl2lacZ/+ conceptuses, counterstained with DAPI (blue). Scale bar: 0.1 mm.