Additional file 4: of A suppressive role of guanine nucleotide-binding protein subunit beta-4 inhibited by DNA methylation in the growth of anti-estrogen resistant breast cancer cells

Figure S3. Knockdown of GNB4 using siRNA suppresses proliferation of parental S05 cells. S05 cells grown to 80% confluency were transiently transfected with either 30 nM GNB4 siRNA or 30 nM negative control siRNA; at 24 h after transfection, the cells were replated in 96-well plate, MTT assay was performed as described in “Methods”. Asterisk indicates p < 0.05. (PPTX 37 kb