Relative comparison of survival motor neuron (SMN) protein expression between the control and spinal muscular atrophy (SMA) subjects.

Peripheral blood nuclear cells (PBCs) classified according to types (R2, R3, R4, R5)were analyzed for the levels of SMN protein expressions in (A) intracellular and (B) intranuclear cells. (C–E) SMN protein spot analysis. (C) The percentage of SMN spot+ cells is equal to “the percentage of spot+ cells in SMN-stained cells” minus “the percentage of spot+ cells in isotype control-stained cells”. The percentage of spot+ cells of isotype control-stained cells was + cells in the CD33++ cell (R4). Cells were obtained from 14 control subjects (open symbols) and 25 SMA subjects (closed symbols). Circles indicate infant subjects and triangles indicate adult subjects. ***p S2 Fig online.