Localization of the survival motor neuron (SMN) proteins with Smith core proteins (Sm) in CD33⁺⁺ cells.

Cells were co-stained with 2B1 and anti-Sm mAb (Y12). The cells shown were extracted from SMN-spot+ CD33++ cells. Data were obtained from two representative individuals; control(C8, 7y; left) and SMA (S7, 5y, right) subjects among the eight examined. The eight-cells were extracted from the SMN spot with the highest intensity. The number in the upper left of the brightfield represents the individual number, depending on the detection order. Images are shown as bright field, nucleus (blue), SMN (green), Sm (red), and merged (yellow).