hg-UNC-25 synthesizes GABA in <i>H</i>. <i>glycines</i>.
- Ziduan Han (5638856)
- Sita Thapa (5638859)
- Ursula Reuter-Carlson (5638862)
- Hannah Reed (5638865)
- Michael Gates (3311481)
- Kris N. Lambert (579211)
- Nathan E. Schroeder (1452250)
Publication date
August 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
The hg-unc-25 cDNA from H. glycines partially rescues the C. elegans unc-25(e156) mutant. In wild-type C. elegans, GABAergic neurons are detected in the head and ventral nerve cord (arrowheads) using anti-GABA staining. In the C. elegans unc-25(e156) mutant, GABA is not produced and GABAergic neurons cannot be detected using anti-GABA staining. A plasmid construct containing hg-unc-25 cDNA driven by the C. elegans unc-25 promotor partially rescues the C. elegans unc-25(e156) mutant as seen by GABA-immunoreactivity (arrowheads). Arrows indicate the expression of coel::RPF used as a co-injection marker. Scale bars, 10 μm.</p
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