Additional file 3 of Hexokinase 2 is dispensable for T cell-dependent immunity

Figure S3. Example Gating Strategies. (A) Example live CD8+ T cell gating, and subsequent gating for evaluation of activation markers. Similar gating used for CD4+ T cells (B) Gating on single cells for mixed populations of CD4+ and CD8+ T cells followed by gating for CD62L and CD44 expression (performed on CD4+ and CD8+ T cells separately). (C) Gating on live CD4+ cells for expression of transcription factors. (D) Gating on CD8+ cells for gp-33 tetramer. (E) Gating on CD4+ cells for YFP. (F) Gating on single cells for CD45.2 and GFP expression. (PDF 1300Â kb