<i>Nematostella</i> Dicer1 and PIWI2 knockdown affects sRNA biogenesis.

In the scatter plots in panels A, C, E and G the sRNA read counts were normalized, each dot represents the abundance of an individual sRNA analyzed from morphants vs. control. sRNAs that showed a depletion greater than two fold are indicated in green. (A) miRNAs in Dicer1 MO vs. control MO (B) Relative abundance of Nematostella miRNAs between Dicer1 MO and control MO, in Dicer1 MO the miRNA read counts were significantly reduced (P Nematostella piRNAs between Dicer1 MO and control MO, in Dicer1 MO the piRNA read counts were not significantly reduced (P = 0.32218, Wilcoxon signed-rank test). (E) miRNAs in Piwi2 MO vs. control. (F) Relative abundance of Nematostella miRNAs between Piwi2 MO and control MO, in Piwi2 MO the miRNA read counts were significantly reduced (P Nematostella piRNAs between Piwi2 MO and control MO, in Piwi2 MO the piRNA read counts were significantly reduced (P < 0.0001, Wilcoxon signed-rank test).