p38 phosphorylation in infected macrophages in presence of DMS, S1P or both in hMDM.

hMDM were cultured in six-well plates in the presence or absence of L. donovani infection (MOI = 1:10) for 6 h, hMDM were washed to remove non-internalized parasites and incubated for next 42 h in presence and absence of S1P, DMS or both. Western Blot showing A. phospho-p38 and total p38, B. Densitometric analysis of phospho-p38 in presence of DMS, S1P, or both in IM, after normalization with total p38. The data is a representation of mean ± SD from two independent experiments *, p< 0.05.