Direct and bystander DC cytokine secretomes.

A) Schema showing how DCs were activated with LPS, HAdV5, IgG, and IC-HAdV5 and when the cell supernatants were harvested. B) Cytokine secretion from IgG-, HAdV5-, dose-dependent IC-HAdV5-, and LPS-challenged DCs at 12 h. C) Cytokine secretion from IgG-, HAdV5-, dose-dependent IC-HAdV5-, and LPS-challenged DCs at 12–21 h. D) Cytokine secretion from bystander DCs (the stimulus used to challenge the direct DCs is above each column at 12–21 h). The color code shows relative increases compared to IgG-challenged-DCs (raw values in first column). The assays were performed twice in duplicate with similar results.