ARID1A protein expression in urothelial carcinomas of different stage and grade compared to normal urothelium of the urinary bladder.

(A-F) Immunohistochemical images representative of observed median ARID1A protein expression in normal urothelium of the urinary bladder (NU, n = 21) (A), pTa low-grade (pTa LG, n = 62) (B) and pTa high-grade (pTa HG, n = 30) tumors (C), carcinoma in situ (CIS, n = 175) (D), pT1 high-grade (pT1 HG, n = 31) (E) and muscle-invasive bladder cancers (MIBC, n = 64) (F). Arrows in A: CIS. Bar: 100μm. (G) Quantification of ARID1A protein expression by box plot analysis. Horizontal lines/numbers: grouped medians. Boxes: 25–75% quartiles. Vertical lines: range, minimum and maximum. Only significant differences are shown. * P < 0.05, ** P <0.01, *** P < 0.001 (Kruskal-Wallis-test, Dunn’s multiple comparison post test), IRS: immunoreactive score. (H) Quantification of ARID1A protein expression loss (IRS = 0–2).