Figure S1. Generation of the Pax7EGFP mouse. (a) Schematic of the Pax7-EGFP targeting BAC that was used to generate the Pax7EGFP line. (b) Agarose gel depicting PCR-genotyped mouse lines from Table 1. A mouse carrying the Pax7EGFP transgene yields a single product of 706 bp. Control (wild-type) mice produce no band at this size. (PDF 442 kb
Figure S3. A, B. Examples of mutagenized loxP sites identified upon TA cloning PCR products from lox...
Figure S4. Identification of Pax7-positive satellite cells by flow cytometry. A. Representative dot ...
Figure S1. ΔKlotho mice display signs of sarcopenia. (A) Immunofluorescence of cross-sections of TA ...
Figure S3. MuSCs from Pax7EGFP mice are similar to Pax7-labeled MuSCs. (a) MuSCs were isolated as in...
Figure S2. Pax7EGFP copy number variation analysis and in-depth genotyping, and effect of transgene ...
Figure S7. Histological analysis of additional Pax7EGFP muscles. Representative images of quadriceps...
Figure S4. FACS schematic of MuSC isolation. Top: gating strategy for the gate selection of parent p...
Figure S6. Technical immunofluorescence control for Fig. 4b. (Left) Pax7EGFP negative mouse TA musc...
Figure S5. Analysis of MuSC proliferation and cell death. (a) Measurement of proliferative capacity ...
Figure S8. Overlap of Pax7+ and GFP+ cells during regeneration. (a) Imaging of tibialis anterior cry...
Table S1. Electroporation parameters obtained by NEPA21 electroporator (NEPAGENE) for gene transduct...
Figure S1. Screening by Sanger sequencing of animals for the generation of a Syt7 conditional allele...
Schematic view of the sources of genetic variation identified in KO/KI congenic mice. First, the cho...
Figure S1. Generation of SacsR272C mice. (A) Targeting vector was constructed by first cloning the g...
Figure S8. Floxed Serpina3n alleles generated by the electroporation of two gRNA, Cas9 mRNA and lssD...
Figure S3. A, B. Examples of mutagenized loxP sites identified upon TA cloning PCR products from lox...
Figure S4. Identification of Pax7-positive satellite cells by flow cytometry. A. Representative dot ...
Figure S1. ΔKlotho mice display signs of sarcopenia. (A) Immunofluorescence of cross-sections of TA ...
Figure S3. MuSCs from Pax7EGFP mice are similar to Pax7-labeled MuSCs. (a) MuSCs were isolated as in...
Figure S2. Pax7EGFP copy number variation analysis and in-depth genotyping, and effect of transgene ...
Figure S7. Histological analysis of additional Pax7EGFP muscles. Representative images of quadriceps...
Figure S4. FACS schematic of MuSC isolation. Top: gating strategy for the gate selection of parent p...
Figure S6. Technical immunofluorescence control for Fig. 4b. (Left) Pax7EGFP negative mouse TA musc...
Figure S5. Analysis of MuSC proliferation and cell death. (a) Measurement of proliferative capacity ...
Figure S8. Overlap of Pax7+ and GFP+ cells during regeneration. (a) Imaging of tibialis anterior cry...
Table S1. Electroporation parameters obtained by NEPA21 electroporator (NEPAGENE) for gene transduct...
Figure S1. Screening by Sanger sequencing of animals for the generation of a Syt7 conditional allele...
Schematic view of the sources of genetic variation identified in KO/KI congenic mice. First, the cho...
Figure S1. Generation of SacsR272C mice. (A) Targeting vector was constructed by first cloning the g...
Figure S8. Floxed Serpina3n alleles generated by the electroporation of two gRNA, Cas9 mRNA and lssD...
Figure S3. A, B. Examples of mutagenized loxP sites identified upon TA cloning PCR products from lox...
Figure S4. Identification of Pax7-positive satellite cells by flow cytometry. A. Representative dot ...
Figure S1. ΔKlotho mice display signs of sarcopenia. (A) Immunofluorescence of cross-sections of TA ...