Immunogenicity of r<i>Tm</i>-WAP-F8+<i>Na</i>-GST-1 and r<i>Tm</i>-CAP-1 proteins in a murine model.

(A) Vaccination schedule to evaluate immunogenicity in male AKR/J mice. Humoral immune response was measured by end-point serum titer for antigen-specific (B) IgG1 and (C) IgG2a by ELISA to calculate the (D) IgG1 to IgG2a ratio for the mice in each group. Mice in groups injected with PBS or Montanide ISA 720 adjuvant were used as negative controls for each coating antigen. Statistical significance: *pTm-WAP-F8+Na-GST-1 (10 μg/mL), rTm-CAP-1 (10 μg/mL), or Tm-ES (50 μg/mL), for 72 hours. Values for cells cultured in media only were subtracted as background from those of cells stimulated with cognate antigens in the corresponding groups of mice. Values from duplicate wells for each treatment/stimulation were averaged for individual cytokines for the 5 mice in each group to calculate statistical significance. Individual cytokines and statistics are shown in S1 Fig.