Additional file 2: of Enhanced IL-6/phosphorylated STAT3 signaling is related to the imbalance of circulating T follicular helper/T follicular regulatory cells in patients with rheumatoid arthritis

Figure S2. Gating strategy describing the expression of PD-1, ICOS, IL-21 and pSTAT3 in CD4+CXCR5+ T cells. CD4+CXCR5+ T cells were gated for determining the expression of PD-1, ICOS, IL-21, and pSTAT3. The cut off for PD-1 (A), ICOS (B), IL-21 (C), or pSTAT3 (D) positivity in CD4+CXCR5+ T cells was determined based on fluorescence minus one (FMO) and isotype control subjects of PD-1, ICOS, IL-21, or pSTAT3. (TIF 240 kb