Additional file 4: of RSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1

Figure S3. Assessment of barrier function in dermal microvascular endothelial monolayers. Uniform tight monolayers of HDMVEC cultured in stabilized and collagen coated 8W10E+ array chambers were treated with RSPO3, IL-1β or a combination of RSPO3 and IL-1β. (A) Original ECIS plot of resistance measurements (line graph) from one representative of three independent experiments run in duplicates at 4000 Hz (indicative of cell-cell adhesion tightness). (B) Bar graph showing the data of barrier function measurements continuously recorded for and at 12 h from three independent experiments run in duplicates. Black, vehicle; Green, IL-1β; Yellow, RSPO3; Purple, RSPO3 + IL-1β. Error bars are mean ± S.D. ***P < 0.001 versus vehicle treatment, ****P < 0.001 versus RSPO3 and IL-1β single treatments. (PDF 242 kb