Changes in movement in response to silent gaps after sham/noise exposure.

Changes in movement in response to silent gaps were measured again after sham/noise exposure (trial 3). These trials were also 8.5 min in duration and contained 6 silent gaps of either 500 ms or 1 s in a background of white noise (80 dB SPL). Movement was again quantified in two ways: 1) “body movement” or movement of the head, tail, and/or torso (center point) and 2) “velocity” or velocity measured at the torso (center point). A-D. Body movement (A and B) is plotted for a mouse that underwent sham (A) and noise (B) exposure. To compare body movement across gaps from these individual trials, data are aligned across gaps (indicated by vertical gray bars) to show body movement for the 30 s preceding the onset of the silent gap, the 5 s containing the gap, and the subsequent 30 s. Because mice were generally very active, body movement plots (A and B) indicate periods of no movement during these time windows. Green traces indicate the average responses across the aligned time windows for the sham (A) and noise (B) exposed mouse. To compare changes in body movement during the silent gaps across mice after sham (C) or noise (D) exposure, relative changes in movement during the 5 s containing the gap and the preceding 30 s were calculated (as described in the Methods) for body movement. Gray dots indicate individual values and black bars indicate mean and SEM. Relative changes in movement were not observed in response to silent gaps following either sham (C) or noise (D) exposure (Kruskal Wallis test with Dunn’s multiple comparisons test). E-H. Velocity (E and F) is plotted for a mouse that underwent sham (E) and noise (F) exposure. To compare velocity across gaps from these individual trials, data are aligned across gaps (indicated by vertical gray bars) to show body movement for the 30 s preceding the onset of the silent gap, the 5 s containing the gap, and the subsequent 30 s. Green traces indicate the average responses across the aligned time windows for the sham (E) and noise (F) exposed mouse. To compare changes in movement during the silent gaps across mice after sham (G) or noise (H) exposure, relative changes in movement during the 5 s containing the gap and the preceding 30 s were calculated (as described in the Methods) for velocity. Gray dots indicate individual values and black bars indicate mean and SEM. Relative changes in velocity were not observed in response to silent gaps following either sham (G) or noise (H) exposure (one-way analysis of variance). Values and original data are provided in Table 3 and S3 Table.