<i>In-vivo</i> studies of Ger on nematode model <i>C</i>. <i>elegans</i>.

(a) Toxicity test of Ger on C. elegans depicted by Kaplan–Meier curve showing % survival of C. elegans in the presence of Ger at sub MIC concentration. Microscopic images (magnification 4x) of nematodes in presence of Ger. Worm survival was determined based on movement (upper panel).The toxicity of Ger was studied on nematodes by determining survival rates after 3 days (lower panel). (b) Ger prolongs the survival of C. albicans infected C. elegans. Microscopic images showing the survival of infected C. elegans when treated with Ger on day 1, day2 and day 3. Kaplan–Meier curve showing % survival of C. albicans infected C. elegans in the presence of Ger (lower panel). (c) Intestinal persistence of C. albicans. Upper panel shows the Act1p-GFP C. albicans visualization confirming lower fungal burden in Ger treated worms contrary to untreated nematodes. Lower panel represents the CFW stained C. albicans visualized in proximal and distal intestine of C. elegans treated with Ger showing lesser fungal burden. (d). Hemolytic activity of Ger depicted as percentage on Y axis in comparison to the positive control Triton X 100.