Contaminated surfaces serve as an important reservoir for Clostridium difficile transmission. Current strategies to detect environmental contamination of C. difficile rely heavily on culture, and often only indicate presence versus absence of spores. The goal of this study was to compare quantitative PCR (qPCR) to culture for the detection and quantification of C. difficile from inert surfaces. First, we compared the limit of detection (LOD) of a 16S rRNA gene and toxin B gene qPCR assay for detection of C. difficile in solution. Second, we compared the LODs of 16S rRNA gene qPCR versus culture for detection of C. difficile from surfaces. Solution experiments were performed by direct seeding of spores into neutralizing broth, whereas surfac...
Clostridium difficile is one of the most important human and animal pathogens. However, the bacteriu...
a<p>“<i>C. difficile</i> positive/negative” was defined by presence/absence of qPCR amplification wi...
The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but...
Contaminated surfaces serve as an important reservoir for Clostridium difficile transmission. Curren...
Clostridioides difficileinfection is an important healthcare-associated infection and inade...
The horizontal transmission of Clostridium difficile in the hospital environment is difficult to est...
Introduction. Environmental surveillance for Clostridioides difficile is challenging. There are no i...
Effective and easy-to-use methods for detecting Clostridium difficile spore contamination would be u...
In the past decade, the incidence of Clostridium difficile infections (CDI) with a more severe cours...
Clostridium difficile is the main cause of nosocomial diarrhea, but is also found in asymptomatic su...
Background: Clostridium difficile is the main cause of nosocomial diarrhea, but is also found in asy...
A retrospective cohort study was performed on Clostridium difficile positive patient isolates and ho...
The recovery of Clostridium difficile spores from hospital surfaces was assessed using rayon swabs, ...
Background: Accurate diagnostic methods for Clostridium difficile infection (CDI) are required for ...
BACKGROUND: In healthcare facilities, Clostridium difficile infections spread by transmission of bac...
Clostridium difficile is one of the most important human and animal pathogens. However, the bacteriu...
a<p>“<i>C. difficile</i> positive/negative” was defined by presence/absence of qPCR amplification wi...
The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but...
Contaminated surfaces serve as an important reservoir for Clostridium difficile transmission. Curren...
Clostridioides difficileinfection is an important healthcare-associated infection and inade...
The horizontal transmission of Clostridium difficile in the hospital environment is difficult to est...
Introduction. Environmental surveillance for Clostridioides difficile is challenging. There are no i...
Effective and easy-to-use methods for detecting Clostridium difficile spore contamination would be u...
In the past decade, the incidence of Clostridium difficile infections (CDI) with a more severe cours...
Clostridium difficile is the main cause of nosocomial diarrhea, but is also found in asymptomatic su...
Background: Clostridium difficile is the main cause of nosocomial diarrhea, but is also found in asy...
A retrospective cohort study was performed on Clostridium difficile positive patient isolates and ho...
The recovery of Clostridium difficile spores from hospital surfaces was assessed using rayon swabs, ...
Background: Accurate diagnostic methods for Clostridium difficile infection (CDI) are required for ...
BACKGROUND: In healthcare facilities, Clostridium difficile infections spread by transmission of bac...
Clostridium difficile is one of the most important human and animal pathogens. However, the bacteriu...
a<p>“<i>C. difficile</i> positive/negative” was defined by presence/absence of qPCR amplification wi...
The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but...