Additional file 1: of Anti-TLR2 antibody triggers oxidative phosphorylation in microglia and increases phagocytosis of β-amyloid

Figure S2. Expression of TLR2 in microglia. Microglia were prepared and treated as described in the legend for Fig. 1 and stained for TLR2 incubating with the primary antibody anti-TLR2 (Abcam, UK) followed by the secondary antibody Alexa Fluor® 488 donkey anti-mouse IgG (1:1000) and mounted in ProLong®Gold with the nuclear marker DAPI (Thermo Scientific, USA). The panel shows fluorescence images at × 40 magnification and shows that TLR2 staining is confined to the membrane of the microglial cells independently of the experimental groups observed. (Scale bar = 50 μm). (PNG 694 kb