Additional file 4: of Cyclin D1-CDK4 activity drives sensitivity to bortezomib in mantle cell lymphoma by blocking autophagy-mediated proteolysis of NOXA

Figure S3. Double knockdown of cyclin D1 and CDK4 antagonizes bortezomib-induced cell death similar to single knockdown of CDK4. MCL cell line Mino was transfected with siRNA targeting CCND1, CDK4, or both. Twenty-four hours after transfection, protein expression was analyzed (right) and cells were treated with 8 nM bortezomib. Cell death was assessed by AnnexinV-PI staining 24 h post-treatment (left). Data represent means ± S.D. from three independent experiments. (TIFF 903 kb