Additional file 7: of Cyclin D1-CDK4 activity drives sensitivity to bortezomib in mantle cell lymphoma by blocking autophagy-mediated proteolysis of NOXA

Figure S6. Palbociclib-mediated antagonism on bortezomib-induced cell death is not caused by alterations in cell cycle distribution. MCL cell line Mino was transfected with siRNA targeting RB1 and treated with 100 nM palbociclib 24 h post-transfection. After 16 h, cells were treated with 8 nM bortezomib. Twenty-four hours after treatment, cell cycle distribution was measured by BrdU staining (left), cell death was assessed by AnnexinV-PI staining (middle panel), and proteins were analyzed by Western blot (right). Data represent means ± S.D. from three independent experiments. (TIFF 802 kb