HOCl signaling is required to promote visceral spasms.

(A) CFU counting in midguts of WT flies fed with 108 CFU of Btk or 108 CFU of Btk + DTT. (B) Measure of midgut length from flies 2 h post-intoxication with DTT (left) or 108 CFU of Btk + DTT (right). (C) Phalloidin staining of posterior midgut 2 h after feeding with 5% sucrose (Ctrl) or Btk + DTT. 20X objective. (D) CFU counting in midguts of WT (grey bars) or myo1Ats>DUOXRNAi (where DUOX expression was silenced in enterocytes, blue bars) flies fed with 108 CFU of Btk. (E) Measure of midgut length of myo1Ats>DUOXRNAi flies 2 h post-intoxication with sucrose (left) or 108 CFU of Btk (right). Note that the silencing of DUOX in enterocytes does not affect the length of midguts compared to those from WT flies fed with 5% sucrose (Ctrl). (F) Phalloidin staining of posterior midgut of myo1Ats>DUOXRNAi flies after feeding with 5% sucrose (left panel) or 108 CFU of Btk (right panel). 20X objective.